Subgingival plaque samples from 259 adult patients with severe periodontitis were analyzed. P. gingivalis was detected in 111 (43%) of the 259 subgingival plaque samples by culture and in 138 (53%) samples by PCR. The sensitivity, speciﬁcity, and positive and negative predictive values of the real-time PCR were 100, 94, 94, and 100%, respectively.

av INIC UNIT · Citerat av 5 — Data collection and instruments . Tooth brushing twice daily to reduce oral debris and dental plaque remains the mainstay of oral health, and gingivitis may serve as a portal for infection and further result in subgingival inflammation and

The site was then cleaned and isolated using cotton roles and air driedgently.AnothersterilizedGraceycurettewasinsertedtothedeepest 2014-08-05 Subgingival plaque samples presented significantly higher counts of Prevotella nigrescens, Prevotella intermedia, Bacteroides forsythus and Porphyromonas gingivalis. Subgingival samples exhibited a significantly higher proportion of “red” and “orange complex” species, while supragingival plaque exhibited higher proportions of “green” and “purple” complex species as well as 2017-11-01 2017-02-03 Sample Collection and Processing. Subgingival plaque samples were collected 1 week after the full-mouth periodontal examination (Feng et al., 2015). All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling. 2020-04-01 Clinical examination of periodontal parameters and collection of saliva and pooled subgingival plaque samples from mesial-buccal sites of 4 first molars were performed before initial therapy and 2, 4 and 6 months respectively after mechanical therapy, and saliva … Subgingival plaque was then collected with sterile paper points (Meta Biomed ® Co. Ltd, Cheongju, South Korea) inserted into the gingival sulcus of Ramfjord’s index teeth for 15 seconds(7-9).

Specific ecologic relation between subgingival microbial species of different virulence initiates gingival inflammation and subsequently causes periodontal destruction. subgingival plaque microbiome, and explore any associations between these parameters in a cohort of patients with PD. Patients with PD only, rather than PD and RA, were chosen to avoid the confounding factors associated with RA-related treatments. Subgingival plaque and the host response to plaque causes periodontal disease. Fermentation of sugar by bacteria in plaque (results in lactate production) causes dental caries (see module 1; Dental Plaque Be Bad for more information on biofilm/plaque formation ).

The purpose of subgingival scaling and root planing is to remove calculus and to smooth the cementum and underlying dentin. This then allows for effective subgingival plaque removal by the patient, which if done daily will help to prevent calculus formation (calculus removal will be discussed in detail in Chap. 5).

Så används Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-83. 32.

A Method for Collection of Subgingival Plaque and Calculus. Herbert I. Oshrain D.D.S. Section of Periodontology and Division of Stomatology, School of Dental and Oral Surgery, Columbia University, New York. Search for more papers by this author. Albert Salkind D.D.S.

Similarly, the presence of subgingival plaque containing gram-positive bacteria may be necessary for its secondary colonization in periodontal pockets. View Show abstract 2015-04-01 · This study suggests subgingival plaque sample collection with curette in order to reduce the uptake of human cells and to restrain the sequencing of human DNA in metagenomic studies performed in high-throughput sequencing platforms. Further investigations with larger sample sizes are encouraged to support these preliminary findings.

Testa NE.se gratis eller Logga in Plaque-induced gingivitis is a gingival inflammation without bone loss. • Periodontitis is an Subgingival debridement using hand and/or ultrasonic instruments. Waerhaug J. Subgingival plaque and loss of attachment in periodontosis as evaluated on extracted teeth.
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We collected samples by inserting 15 sterile endodontic paper-points (Caulk-Dentsply, Milford, DE, USA) into the pockets for 10 sec, following isolation and supragingival plaque removal. plaque, subgingival plaque samples were collected immedi-ately from the labial or buccal surfaces of index teeth with a sterilized periodontal curette. All samples were pooled together into a microcentrifuge tube that contained 1.0 mL of sterilized saline water and were stored immediately at − 80 °C. DNA Isolation and 16S rRNA Gene Sequencing The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria.

Gingivitis does NOT occur following any specific pathogen being present in the dental biofilm/plaque.
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2016-01-01

The food you eat, traditional techniques lead to soft tissue damage (gums), and subgingival restorative margins (leaving of foreign materials (margins) below the gum), which 2- Sampling: After removal of supragingival plaque by means of sterile cotton balls, subgingival plaque was collected on Gracey curette inserted to the depth of 13 Oct 2016 Subgingival plaque and the host response to plaque causes A diverse collection of oral microorganisms are found in dental biofilms.